Stability of hepatitis C virus RNA in blood samples by TaqMan real-time PCR
نویسندگان
چکیده
منابع مشابه
The Study of Cerumen Hepatitis B Infection in Chronic Hepatitis B Patients by Real-Time PCR
Introduction: The hepatitis B is a viral infection that causes a big problem globally. About 2 billion people worldwide are infected and there are now about 400 million HBV-DNA carriers around the world. HBV infection is the ninth cause of death worldwide and infects about 350 million new cases each year in the world. HBV-DNA can be spotted in different body secretions and fluids, including se...
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Information on the concentration of hepatitis C virus (HCV) RNA is useful for patient management, including assessing treatment initiation and treatment response, and monitoring follow-up. Previous studies have reported that poor sample processing and storage conditions might influence the stability of HCV RNA, and hence its detectability. We analyzed 30 patients known to be positive with high/...
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conclusions the quite sensitive in-house taqman real time rt-pcr assay was able to detect and quantify all four main hcv genotypes prevailing around all geographical regions of iran. results the lower limit detection of this in-house hcv real-time rt-pcr was determined as 100 rna copies/ml. inter- and intra-assay coefficient of variation (cv) of this in-house hcv real-time rt-pcr ranged from 0....
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A newly developed real-time RT-polymerase chain reaction assay for quantitation of hepatitis C virus (HCV) RNA in human plasma and serum was applied. A pair of primers and a probe (molecular beacon) were designed that are specific for the recognition of a highly conservative 5'-non-coding region (5'-NCR) in HCV genome. HCV real-time RT-PCR assay had a sensitivity of 1000 RNA copies per reaction...
متن کاملHigh-throughput real-time reverse transcription-PCR quantitation of hepatitis C virus RNA.
We describe a rapid and reproducible method for assessment of the hepatitis C virus (HCV) load in serum samples. The method combines Taqman technology (Roche) and the ABI Prism 7700 (Perkin Elmer) real-time sequence detection system. We have optimized a single-tube reverse transcription-PCR (RT-PCR) that contains a dual-labeled fluorogenic probe to quantify the 5' noncoding region (5' NCR) of H...
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ژورنال
عنوان ژورنال: Journal of Clinical Laboratory Analysis
سال: 2010
ISSN: 0887-8013,1098-2825
DOI: 10.1002/jcla.20354